我就废话不多说了，直接上代码吧！
# -*- coding: UTF-8 -*-
import os
import re
port = 8080
def kill_process():
ret = os.popen("netstat -nao|findstr " + str(port))
#注意解码方式和cmd要相同，即为"gbk"，否则输出乱码
str_list = ret.read().decode('gbk')
ret_list = re.split('',str_list)
try:
process_pid = list(ret_list[0].split())[-1]
os.popen('taskkill /pid ' + str(process_pid) + ' /F')
print "端口已被释放"
except:
print "端口未被使用"
if __name__ == '__main__':
kill_process()
# output1 = os.popen('ipconfig')
# print output1.read().decode('gbk')